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4x protein sample loading buffer  (LI-COR)


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    Structured Review

    LI-COR 4x protein sample loading buffer
    4x Protein Sample Loading Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+protein+sample+loading+buffer/PROTEIN+LOADING+BUFFER/bio_rxiv__64898__2026__03__10__710773-136-8-13
    Average 96 stars, based on 483 article reviews
    4x protein sample loading buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Anellovirus protein encoded by ORF2/3 functions as the viral replication initiation protein.
    Article Snippet: ORF2 and ORF2/3 antibodies were conjugated to M- 270 Epoxy beads (Thermo Fisher #14311D) for FLAG IPs, and ANTI- FLAG® M2 Magnetic Beads (Sigma # M8823) were used for ORF2/3 IPs. .. Cell lysates were incubated with antibody- bead slurries overnight at 4C, beads were pelleted, washed twice with cold lysis buffer, and proteins were eluted using 4X Protein Sample Loading buffer (LI- COR). .. Beads were incubated in elution buffer at 65C for 20 min, separated from the eluate, and stored at −80 °C until quantitative proteomic sample preparation and analysis (performed at IQ Proteomics, Cambridge, MA).

    Article Title: Characterizing and engineering post-translational modifications with high-throughput cell-free expression
    Article Snippet: .. Following incubation, 1 μL of 1 M DTT, 2.5 μL of 4X Protein Sample Loading Buffer for Western Blots (Li-COR Biosciences), and 2.5 μL of water were added to each sample and the samples were then incubated at 70 °C for 10 min. ..

    Lysis:

    Article Title: Anellovirus protein encoded by ORF2/3 functions as the viral replication initiation protein.
    Article Snippet: ORF2 and ORF2/3 antibodies were conjugated to M- 270 Epoxy beads (Thermo Fisher #14311D) for FLAG IPs, and ANTI- FLAG® M2 Magnetic Beads (Sigma # M8823) were used for ORF2/3 IPs. .. Cell lysates were incubated with antibody- bead slurries overnight at 4C, beads were pelleted, washed twice with cold lysis buffer, and proteins were eluted using 4X Protein Sample Loading buffer (LI- COR). .. Beads were incubated in elution buffer at 65C for 20 min, separated from the eluate, and stored at −80 °C until quantitative proteomic sample preparation and analysis (performed at IQ Proteomics, Cambridge, MA).

    Article Title: Replication-competent SIVcpz CRISPR screen identifies barriers to successful cross-species transmission
    Article Snippet: .. The viral pellet was then lysed in Western blot lysis buffer with 4x Protein Sample Loading Buffer (LICOR) supplemented with 10% β-mercaptoethanol (Sigma Aldrich) and heated at 95°C for 5 min. ..

    Marker:

    Article Title: Modulation of mitochondria-ER contacts decrease inflammasome formation and restores amyloid β-peptide phagocytosis in adult mouse microglia
    Article Snippet: .. Equal amounts of protein (15-20 μg) mixed with 4x Protein Sample Loading Buffer (LI-COR Biosciences, #92840004) and Amersham TM ECL TM Rainbow TM Marker - Full range (Cytiva, #GERPN800E) were separated by SDS-PAGE using NuPAGETM Bis-Tris Mini Protein Gels, 4–12% (Invitrogen) or NuPAGETM Tris-Acetate Mini Protein Gels, 7% (used exclusively for IP3R detection; Invitrogen). .. Proteins were transferred onto AmershamTM Protran 0.45 μm nitrocellulose membrane (Cytiva).

    Article Title: TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions
    Article Snippet: .. SDS-PAGEs were performed with NuPAGETM 4–12% Bis-Tris Midi Protein Gels (Thermo Fisher Scientific Inc.) in a CriterionTM Cell electrophoresis chamber (Bio-Rad Laboratories, Inc.) using NuPAGETM MES SDS as running buffer, Chameleon® Duo Pre-stained Proteinmaker (LI-COR Environmental) as size marker and 4x Protein Sample Loading Buffer (LI-COR Environmental) with added β -mercaptoethanol. .. Electrophoresis was performed with a PowerPac® 200 (Bio-Rad Laboratories) at 160 V. Silver nitrate staining of gels was executed as described in .

    SDS Page:

    Article Title: Modulation of mitochondria-ER contacts decrease inflammasome formation and restores amyloid β-peptide phagocytosis in adult mouse microglia
    Article Snippet: .. Equal amounts of protein (15-20 μg) mixed with 4x Protein Sample Loading Buffer (LI-COR Biosciences, #92840004) and Amersham TM ECL TM Rainbow TM Marker - Full range (Cytiva, #GERPN800E) were separated by SDS-PAGE using NuPAGETM Bis-Tris Mini Protein Gels, 4–12% (Invitrogen) or NuPAGETM Tris-Acetate Mini Protein Gels, 7% (used exclusively for IP3R detection; Invitrogen). .. Proteins were transferred onto AmershamTM Protran 0.45 μm nitrocellulose membrane (Cytiva).

    other:

    Article Title: An IMPDH2 variant associated with neurodevelopmental disorder disrupts purine biosynthesis and somite organization.
    Article Snippet: IMPDH2 is a critical enzyme for de novo purine biosynthesis, regulating the balance between adenine and guanine nucleotides.. Under purine stress, it forms filaments that resist feedback inhibition by GTP.. Patients with gain-of-function variants of this enzyme suffer from early-onset neuromotor symptoms including dystonia.

    Electrophoresis:

    Article Title: TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions
    Article Snippet: .. SDS-PAGEs were performed with NuPAGETM 4–12% Bis-Tris Midi Protein Gels (Thermo Fisher Scientific Inc.) in a CriterionTM Cell electrophoresis chamber (Bio-Rad Laboratories, Inc.) using NuPAGETM MES SDS as running buffer, Chameleon® Duo Pre-stained Proteinmaker (LI-COR Environmental) as size marker and 4x Protein Sample Loading Buffer (LI-COR Environmental) with added β -mercaptoethanol. .. Electrophoresis was performed with a PowerPac® 200 (Bio-Rad Laboratories) at 160 V. Silver nitrate staining of gels was executed as described in .

    Western Blot:

    Article Title: Replication-competent SIVcpz CRISPR screen identifies barriers to successful cross-species transmission
    Article Snippet: .. The viral pellet was then lysed in Western blot lysis buffer with 4x Protein Sample Loading Buffer (LICOR) supplemented with 10% β-mercaptoethanol (Sigma Aldrich) and heated at 95°C for 5 min. ..

    Article Title: Characterizing and engineering post-translational modifications with high-throughput cell-free expression
    Article Snippet: .. Following incubation, 1 μL of 1 M DTT, 2.5 μL of 4X Protein Sample Loading Buffer for Western Blots (Li-COR Biosciences), and 2.5 μL of water were added to each sample and the samples were then incubated at 70 °C for 10 min. ..



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